Release of severe acute respiratory syndrome coronavirus nuclear import block enhances host transcription in human lung cells.
Identifieur interne : 001871 ( Main/Exploration ); précédent : 001870; suivant : 001872Release of severe acute respiratory syndrome coronavirus nuclear import block enhances host transcription in human lung cells.
Auteurs : Amy C. Sims [États-Unis] ; Susan C. Tilton ; Vineet D. Menachery ; Lisa E. Gralinski ; Alexandra Sch Fer ; Melissa M. Matzke ; Bobbie-Jo M. Webb-Robertson ; Jean Chang ; Maria L. Luna ; Casey E. Long ; Anil K. Shukla ; Armand R. Bankhead ; Susan E. Burkett ; Gregory Zornetzer ; Chien-Te Kent Tseng ; Thomas O. Metz ; Raymond Pickles ; Shannon Mcweeney ; Richard D. Smith ; Michael G. Katze ; Katrina M. Waters ; Ralph S. BaricSource :
- Journal of virology [ 1098-5514 ] ; 2013.
Descripteurs français
- KwdFr :
- Amorces ADN (génétique), Analyse sur microréseau, Biologie des systèmes (), Biologie informatique (), Cellules épithéliales (métabolisme), Cellules épithéliales (virologie), Humains, Immunoprécipitation de la chromatine, Poumon (cytologie), Protéines virales régulatrices ou accessoires (métabolisme), Protéomique, Réaction de polymérisation en chaine en temps réel, Réseaux de régulation génique (physiologie), Transcription génétique (physiologie), Transduction du signal (physiologie), Transport nucléaire actif (physiologie), Virus du SRAS (métabolisme).
- MESH :
- cytologie : Poumon.
- génétique : Amorces ADN.
- métabolisme : Cellules épithéliales, Protéines virales régulatrices ou accessoires, Virus du SRAS.
- physiologie : Réseaux de régulation génique, Transcription génétique, Transduction du signal, Transport nucléaire actif.
- virologie : Cellules épithéliales.
- Analyse sur microréseau, Biologie des systèmes, Biologie informatique, Humains, Immunoprécipitation de la chromatine, Protéomique, Réaction de polymérisation en chaine en temps réel.
English descriptors
- KwdEn :
- Active Transport, Cell Nucleus (physiology), Chromatin Immunoprecipitation, Computational Biology (methods), DNA Primers (genetics), Epithelial Cells (metabolism), Epithelial Cells (virology), Gene Regulatory Networks (physiology), Humans, Lung (cytology), Microarray Analysis, Proteomics, Real-Time Polymerase Chain Reaction, SARS Virus (metabolism), Signal Transduction (physiology), Systems Biology (methods), Transcription, Genetic (physiology), Viral Regulatory and Accessory Proteins (metabolism).
- MESH :
- chemical , genetics : DNA Primers.
- cytology : Lung.
- metabolism : Epithelial Cells, SARS Virus, Viral Regulatory and Accessory Proteins.
- methods : Computational Biology, Systems Biology.
- physiology : Active Transport, Cell Nucleus, Gene Regulatory Networks, Signal Transduction, Transcription, Genetic.
- virology : Epithelial Cells.
- Chromatin Immunoprecipitation, Humans, Microarray Analysis, Proteomics, Real-Time Polymerase Chain Reaction.
Abstract
The severe acute respiratory syndrome coronavirus accessory protein ORF6 antagonizes interferon signaling by blocking karyopherin-mediated nuclear import processes. Viral nuclear import antagonists, expressed by several highly pathogenic RNA viruses, likely mediate pleiotropic effects on host gene expression, presumably interfering with transcription factors, cytokines, hormones, and/or signaling cascades that occur in response to infection. By bioinformatic and systems biology approaches, we evaluated the impact of nuclear import antagonism on host expression networks by using human lung epithelial cells infected with either wild-type virus or a mutant that does not express ORF6 protein. Microarray analysis revealed significant changes in differential gene expression, with approximately twice as many upregulated genes in the mutant virus samples by 48 h postinfection, despite identical viral titers. Our data demonstrated that ORF6 protein expression attenuates the activity of numerous karyopherin-dependent host transcription factors (VDR, CREB1, SMAD4, p53, EpasI, and Oct3/4) that are critical for establishing antiviral responses and regulating key host responses during virus infection. Results were confirmed by proteomic and chromatin immunoprecipitation assay analyses and in parallel microarray studies using infected primary human airway epithelial cell cultures. The data strongly support the hypothesis that viral antagonists of nuclear import actively manipulate host responses in specific hierarchical patterns, contributing to the viral pathogenic potential in vivo. Importantly, these studies and modeling approaches not only provide templates for evaluating virus antagonism of nuclear import processes but also can reveal candidate cellular genes and pathways that may significantly influence disease outcomes following severe acute respiratory syndrome coronavirus infection in vivo.
DOI: 10.1128/JVI.02520-12
PubMed: 23365422
Affiliations:
- États-Unis
- Caroline du Nord
- Chapel Hill (Caroline du Nord)
- Université de Caroline du Nord à Chapel Hill
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 001257
- to stream PubMed, to step Curation: 001257
- to stream PubMed, to step Checkpoint: 001112
- to stream Ncbi, to step Merge: 002614
- to stream Ncbi, to step Curation: 002614
- to stream Ncbi, to step Checkpoint: 002614
- to stream Main, to step Merge: 001877
- to stream Main, to step Curation: 001871
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Release of severe acute respiratory syndrome coronavirus nuclear import block enhances host transcription in human lung cells.</title><author><name sortKey="Sims, Amy C" sort="Sims, Amy C" uniqKey="Sims A" first="Amy C" last="Sims">Amy C. Sims</name><affiliation wicri:level="4"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA. sims0018@ad.unc.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea><placeName><region type="state">Caroline du Nord</region><settlement type="city">Chapel Hill (Caroline du Nord)</settlement></placeName><orgName type="university">Université de Caroline du Nord à Chapel Hill</orgName></affiliation></author><author><name sortKey="Tilton, Susan C" sort="Tilton, Susan C" uniqKey="Tilton S" first="Susan C" last="Tilton">Susan C. Tilton</name></author><author><name sortKey="Menachery, Vineet D" sort="Menachery, Vineet D" uniqKey="Menachery V" first="Vineet D" last="Menachery">Vineet D. Menachery</name></author><author><name sortKey="Gralinski, Lisa E" sort="Gralinski, Lisa E" uniqKey="Gralinski L" first="Lisa E" last="Gralinski">Lisa E. Gralinski</name></author><author><name sortKey="Sch Fer, Alexandra" sort="Sch Fer, Alexandra" uniqKey="Sch Fer A" first="Alexandra" last="Sch Fer">Alexandra Sch Fer</name></author><author><name sortKey="Matzke, Melissa M" sort="Matzke, Melissa M" uniqKey="Matzke M" first="Melissa M" last="Matzke">Melissa M. Matzke</name></author><author><name sortKey="Webb Robertson, Bobbie Jo M" sort="Webb Robertson, Bobbie Jo M" uniqKey="Webb Robertson B" first="Bobbie-Jo M" last="Webb-Robertson">Bobbie-Jo M. Webb-Robertson</name></author><author><name sortKey="Chang, Jean" sort="Chang, Jean" uniqKey="Chang J" first="Jean" last="Chang">Jean Chang</name></author><author><name sortKey="Luna, Maria L" sort="Luna, Maria L" uniqKey="Luna M" first="Maria L" last="Luna">Maria L. Luna</name></author><author><name sortKey="Long, Casey E" sort="Long, Casey E" uniqKey="Long C" first="Casey E" last="Long">Casey E. Long</name></author><author><name sortKey="Shukla, Anil K" sort="Shukla, Anil K" uniqKey="Shukla A" first="Anil K" last="Shukla">Anil K. Shukla</name></author><author><name sortKey="Bankhead, Armand R" sort="Bankhead, Armand R" uniqKey="Bankhead A" first="Armand R" last="Bankhead">Armand R. Bankhead</name></author><author><name sortKey="Burkett, Susan E" sort="Burkett, Susan E" uniqKey="Burkett S" first="Susan E" last="Burkett">Susan E. Burkett</name></author><author><name sortKey="Zornetzer, Gregory" sort="Zornetzer, Gregory" uniqKey="Zornetzer G" first="Gregory" last="Zornetzer">Gregory Zornetzer</name></author><author><name sortKey="Tseng, Chien Te Kent" sort="Tseng, Chien Te Kent" uniqKey="Tseng C" first="Chien-Te Kent" last="Tseng">Chien-Te Kent Tseng</name></author><author><name sortKey="Metz, Thomas O" sort="Metz, Thomas O" uniqKey="Metz T" first="Thomas O" last="Metz">Thomas O. Metz</name></author><author><name sortKey="Pickles, Raymond" sort="Pickles, Raymond" uniqKey="Pickles R" first="Raymond" last="Pickles">Raymond Pickles</name></author><author><name sortKey="Mcweeney, Shannon" sort="Mcweeney, Shannon" uniqKey="Mcweeney S" first="Shannon" last="Mcweeney">Shannon Mcweeney</name></author><author><name sortKey="Smith, Richard D" sort="Smith, Richard D" uniqKey="Smith R" first="Richard D" last="Smith">Richard D. Smith</name></author><author><name sortKey="Katze, Michael G" sort="Katze, Michael G" uniqKey="Katze M" first="Michael G" last="Katze">Michael G. Katze</name></author><author><name sortKey="Waters, Katrina M" sort="Waters, Katrina M" uniqKey="Waters K" first="Katrina M" last="Waters">Katrina M. Waters</name></author><author><name sortKey="Baric, Ralph S" sort="Baric, Ralph S" uniqKey="Baric R" first="Ralph S" last="Baric">Ralph S. Baric</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:23365422</idno><idno type="pmid">23365422</idno><idno type="doi">10.1128/JVI.02520-12</idno><idno type="wicri:Area/PubMed/Corpus">001257</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001257</idno><idno type="wicri:Area/PubMed/Curation">001257</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001257</idno><idno type="wicri:Area/PubMed/Checkpoint">001112</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001112</idno><idno type="wicri:Area/Ncbi/Merge">002614</idno><idno type="wicri:Area/Ncbi/Curation">002614</idno><idno type="wicri:Area/Ncbi/Checkpoint">002614</idno><idno type="wicri:Area/Main/Merge">001877</idno><idno type="wicri:Area/Main/Curation">001871</idno><idno type="wicri:Area/Main/Exploration">001871</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Release of severe acute respiratory syndrome coronavirus nuclear import block enhances host transcription in human lung cells.</title><author><name sortKey="Sims, Amy C" sort="Sims, Amy C" uniqKey="Sims A" first="Amy C" last="Sims">Amy C. Sims</name><affiliation wicri:level="4"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA. sims0018@ad.unc.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea><placeName><region type="state">Caroline du Nord</region><settlement type="city">Chapel Hill (Caroline du Nord)</settlement></placeName><orgName type="university">Université de Caroline du Nord à Chapel Hill</orgName></affiliation></author><author><name sortKey="Tilton, Susan C" sort="Tilton, Susan C" uniqKey="Tilton S" first="Susan C" last="Tilton">Susan C. Tilton</name></author><author><name sortKey="Menachery, Vineet D" sort="Menachery, Vineet D" uniqKey="Menachery V" first="Vineet D" last="Menachery">Vineet D. Menachery</name></author><author><name sortKey="Gralinski, Lisa E" sort="Gralinski, Lisa E" uniqKey="Gralinski L" first="Lisa E" last="Gralinski">Lisa E. Gralinski</name></author><author><name sortKey="Sch Fer, Alexandra" sort="Sch Fer, Alexandra" uniqKey="Sch Fer A" first="Alexandra" last="Sch Fer">Alexandra Sch Fer</name></author><author><name sortKey="Matzke, Melissa M" sort="Matzke, Melissa M" uniqKey="Matzke M" first="Melissa M" last="Matzke">Melissa M. Matzke</name></author><author><name sortKey="Webb Robertson, Bobbie Jo M" sort="Webb Robertson, Bobbie Jo M" uniqKey="Webb Robertson B" first="Bobbie-Jo M" last="Webb-Robertson">Bobbie-Jo M. Webb-Robertson</name></author><author><name sortKey="Chang, Jean" sort="Chang, Jean" uniqKey="Chang J" first="Jean" last="Chang">Jean Chang</name></author><author><name sortKey="Luna, Maria L" sort="Luna, Maria L" uniqKey="Luna M" first="Maria L" last="Luna">Maria L. Luna</name></author><author><name sortKey="Long, Casey E" sort="Long, Casey E" uniqKey="Long C" first="Casey E" last="Long">Casey E. Long</name></author><author><name sortKey="Shukla, Anil K" sort="Shukla, Anil K" uniqKey="Shukla A" first="Anil K" last="Shukla">Anil K. Shukla</name></author><author><name sortKey="Bankhead, Armand R" sort="Bankhead, Armand R" uniqKey="Bankhead A" first="Armand R" last="Bankhead">Armand R. Bankhead</name></author><author><name sortKey="Burkett, Susan E" sort="Burkett, Susan E" uniqKey="Burkett S" first="Susan E" last="Burkett">Susan E. Burkett</name></author><author><name sortKey="Zornetzer, Gregory" sort="Zornetzer, Gregory" uniqKey="Zornetzer G" first="Gregory" last="Zornetzer">Gregory Zornetzer</name></author><author><name sortKey="Tseng, Chien Te Kent" sort="Tseng, Chien Te Kent" uniqKey="Tseng C" first="Chien-Te Kent" last="Tseng">Chien-Te Kent Tseng</name></author><author><name sortKey="Metz, Thomas O" sort="Metz, Thomas O" uniqKey="Metz T" first="Thomas O" last="Metz">Thomas O. Metz</name></author><author><name sortKey="Pickles, Raymond" sort="Pickles, Raymond" uniqKey="Pickles R" first="Raymond" last="Pickles">Raymond Pickles</name></author><author><name sortKey="Mcweeney, Shannon" sort="Mcweeney, Shannon" uniqKey="Mcweeney S" first="Shannon" last="Mcweeney">Shannon Mcweeney</name></author><author><name sortKey="Smith, Richard D" sort="Smith, Richard D" uniqKey="Smith R" first="Richard D" last="Smith">Richard D. Smith</name></author><author><name sortKey="Katze, Michael G" sort="Katze, Michael G" uniqKey="Katze M" first="Michael G" last="Katze">Michael G. Katze</name></author><author><name sortKey="Waters, Katrina M" sort="Waters, Katrina M" uniqKey="Waters K" first="Katrina M" last="Waters">Katrina M. Waters</name></author><author><name sortKey="Baric, Ralph S" sort="Baric, Ralph S" uniqKey="Baric R" first="Ralph S" last="Baric">Ralph S. Baric</name></author></analytic><series><title level="j">Journal of virology</title><idno type="eISSN">1098-5514</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Active Transport, Cell Nucleus (physiology)</term><term>Chromatin Immunoprecipitation</term><term>Computational Biology (methods)</term><term>DNA Primers (genetics)</term><term>Epithelial Cells (metabolism)</term><term>Epithelial Cells (virology)</term><term>Gene Regulatory Networks (physiology)</term><term>Humans</term><term>Lung (cytology)</term><term>Microarray Analysis</term><term>Proteomics</term><term>Real-Time Polymerase Chain Reaction</term><term>SARS Virus (metabolism)</term><term>Signal Transduction (physiology)</term><term>Systems Biology (methods)</term><term>Transcription, Genetic (physiology)</term><term>Viral Regulatory and Accessory Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Amorces ADN (génétique)</term><term>Analyse sur microréseau</term><term>Biologie des systèmes ()</term><term>Biologie informatique ()</term><term>Cellules épithéliales (métabolisme)</term><term>Cellules épithéliales (virologie)</term><term>Humains</term><term>Immunoprécipitation de la chromatine</term><term>Poumon (cytologie)</term><term>Protéines virales régulatrices ou accessoires (métabolisme)</term><term>Protéomique</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Réseaux de régulation génique (physiologie)</term><term>Transcription génétique (physiologie)</term><term>Transduction du signal (physiologie)</term><term>Transport nucléaire actif (physiologie)</term><term>Virus du SRAS (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA Primers</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Poumon</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Lung</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Amorces ADN</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Epithelial Cells</term><term>SARS Virus</term><term>Viral Regulatory and Accessory Proteins</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Computational Biology</term><term>Systems Biology</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cellules épithéliales</term><term>Protéines virales régulatrices ou accessoires</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Réseaux de régulation génique</term><term>Transcription génétique</term><term>Transduction du signal</term><term>Transport nucléaire actif</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Active Transport, Cell Nucleus</term><term>Gene Regulatory Networks</term><term>Signal Transduction</term><term>Transcription, Genetic</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Cellules épithéliales</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Epithelial Cells</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Chromatin Immunoprecipitation</term><term>Humans</term><term>Microarray Analysis</term><term>Proteomics</term><term>Real-Time Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse sur microréseau</term><term>Biologie des systèmes</term><term>Biologie informatique</term><term>Humains</term><term>Immunoprécipitation de la chromatine</term><term>Protéomique</term><term>Réaction de polymérisation en chaine en temps réel</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The severe acute respiratory syndrome coronavirus accessory protein ORF6 antagonizes interferon signaling by blocking karyopherin-mediated nuclear import processes. Viral nuclear import antagonists, expressed by several highly pathogenic RNA viruses, likely mediate pleiotropic effects on host gene expression, presumably interfering with transcription factors, cytokines, hormones, and/or signaling cascades that occur in response to infection. By bioinformatic and systems biology approaches, we evaluated the impact of nuclear import antagonism on host expression networks by using human lung epithelial cells infected with either wild-type virus or a mutant that does not express ORF6 protein. Microarray analysis revealed significant changes in differential gene expression, with approximately twice as many upregulated genes in the mutant virus samples by 48 h postinfection, despite identical viral titers. Our data demonstrated that ORF6 protein expression attenuates the activity of numerous karyopherin-dependent host transcription factors (VDR, CREB1, SMAD4, p53, EpasI, and Oct3/4) that are critical for establishing antiviral responses and regulating key host responses during virus infection. Results were confirmed by proteomic and chromatin immunoprecipitation assay analyses and in parallel microarray studies using infected primary human airway epithelial cell cultures. The data strongly support the hypothesis that viral antagonists of nuclear import actively manipulate host responses in specific hierarchical patterns, contributing to the viral pathogenic potential in vivo. Importantly, these studies and modeling approaches not only provide templates for evaluating virus antagonism of nuclear import processes but also can reveal candidate cellular genes and pathways that may significantly influence disease outcomes following severe acute respiratory syndrome coronavirus infection in vivo.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Caroline du Nord</li></region><settlement><li>Chapel Hill (Caroline du Nord)</li></settlement><orgName><li>Université de Caroline du Nord à Chapel Hill</li></orgName></list><tree><noCountry><name sortKey="Bankhead, Armand R" sort="Bankhead, Armand R" uniqKey="Bankhead A" first="Armand R" last="Bankhead">Armand R. Bankhead</name><name sortKey="Baric, Ralph S" sort="Baric, Ralph S" uniqKey="Baric R" first="Ralph S" last="Baric">Ralph S. Baric</name><name sortKey="Burkett, Susan E" sort="Burkett, Susan E" uniqKey="Burkett S" first="Susan E" last="Burkett">Susan E. Burkett</name><name sortKey="Chang, Jean" sort="Chang, Jean" uniqKey="Chang J" first="Jean" last="Chang">Jean Chang</name><name sortKey="Gralinski, Lisa E" sort="Gralinski, Lisa E" uniqKey="Gralinski L" first="Lisa E" last="Gralinski">Lisa E. Gralinski</name><name sortKey="Katze, Michael G" sort="Katze, Michael G" uniqKey="Katze M" first="Michael G" last="Katze">Michael G. Katze</name><name sortKey="Long, Casey E" sort="Long, Casey E" uniqKey="Long C" first="Casey E" last="Long">Casey E. Long</name><name sortKey="Luna, Maria L" sort="Luna, Maria L" uniqKey="Luna M" first="Maria L" last="Luna">Maria L. Luna</name><name sortKey="Matzke, Melissa M" sort="Matzke, Melissa M" uniqKey="Matzke M" first="Melissa M" last="Matzke">Melissa M. Matzke</name><name sortKey="Mcweeney, Shannon" sort="Mcweeney, Shannon" uniqKey="Mcweeney S" first="Shannon" last="Mcweeney">Shannon Mcweeney</name><name sortKey="Menachery, Vineet D" sort="Menachery, Vineet D" uniqKey="Menachery V" first="Vineet D" last="Menachery">Vineet D. Menachery</name><name sortKey="Metz, Thomas O" sort="Metz, Thomas O" uniqKey="Metz T" first="Thomas O" last="Metz">Thomas O. Metz</name><name sortKey="Pickles, Raymond" sort="Pickles, Raymond" uniqKey="Pickles R" first="Raymond" last="Pickles">Raymond Pickles</name><name sortKey="Sch Fer, Alexandra" sort="Sch Fer, Alexandra" uniqKey="Sch Fer A" first="Alexandra" last="Sch Fer">Alexandra Sch Fer</name><name sortKey="Shukla, Anil K" sort="Shukla, Anil K" uniqKey="Shukla A" first="Anil K" last="Shukla">Anil K. Shukla</name><name sortKey="Smith, Richard D" sort="Smith, Richard D" uniqKey="Smith R" first="Richard D" last="Smith">Richard D. Smith</name><name sortKey="Tilton, Susan C" sort="Tilton, Susan C" uniqKey="Tilton S" first="Susan C" last="Tilton">Susan C. Tilton</name><name sortKey="Tseng, Chien Te Kent" sort="Tseng, Chien Te Kent" uniqKey="Tseng C" first="Chien-Te Kent" last="Tseng">Chien-Te Kent Tseng</name><name sortKey="Waters, Katrina M" sort="Waters, Katrina M" uniqKey="Waters K" first="Katrina M" last="Waters">Katrina M. Waters</name><name sortKey="Webb Robertson, Bobbie Jo M" sort="Webb Robertson, Bobbie Jo M" uniqKey="Webb Robertson B" first="Bobbie-Jo M" last="Webb-Robertson">Bobbie-Jo M. Webb-Robertson</name><name sortKey="Zornetzer, Gregory" sort="Zornetzer, Gregory" uniqKey="Zornetzer G" first="Gregory" last="Zornetzer">Gregory Zornetzer</name></noCountry><country name="États-Unis"><region name="Caroline du Nord"><name sortKey="Sims, Amy C" sort="Sims, Amy C" uniqKey="Sims A" first="Amy C" last="Sims">Amy C. Sims</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001871 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001871 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:23365422
|texte= Release of severe acute respiratory syndrome coronavirus nuclear import block enhances host transcription in human lung cells.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:23365422" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |